Infectious hypodermal and hematopoietic necrosis virus(IHHNV) Nucleic Acid Detection Kits (PCR-Fluorescent Probe Method) Intended use: This kit is suitable for the specific detection of IHHNV in water samples, shrimp seedlings, bait, etc., and is used for the etiological diagnosis of suspected infected persons. Principle: This kit is chosen infectious subcutaneous and hematopoietic organ necrosis virus (IHHNV) specific fragment to design primers probe probe, the probe with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenched group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of infectious subcutaneous and hematopoietic necrosis virus (IHHNV) in a completely closed reaction system. Main components: Component Specification PCR reaction liquid 24 tubes Positive control 1 tube Negative control 1 tube Note: components in different batches of kits should not be interchangeable. Sample requirements: 1. Sample type Seedlings: parent, shrimp eggs, seedlings, coarse standard products Pond: bottom mud, excrement, water body Bait: live bait, chilled bait and non-high-temperature treated bait samples 2. Transport Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw. 3. Sample preservation It can be stored for 24 hours at 2?~8?

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