Description 2xPower Green qPCR mix is designed for high-performance, high-throughput, high-specificity real-time PCR. The kit contains a Hot Start Taq DNA polymerase engineered through a process of molecular evolution. The result is a unique enzyme, specifically designed for qPCR using SYBR® Green I dye chemistry. 2xPower Green qPCR mix is a convenient premix of the components (except primers, template, and water) necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green dye binds to double-stranded (ds) DNA, thus providing a fluorescent signal that reflects the amount of dsDNA product generated during PCR. Applications Gene expression analysis Low copy gene detection Microarray validation Gene knockdown validation Features •Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and tight reproducible amplification. •Highly reproducible CTs over a broad dynamic range. •Compatibility with most real-time qPCR instruments. Composition of the 2x Hy-Power mix 100 mM KCl , 5 mM MgCl2, 400 μM dNTPs, 0.1 U/μl Hot Start Taq DNA Polymerase, 1x SYBR® Green and other optimized buffer components.

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